Development of gut inflammation in mice colonized with mucosa-associated bacteria from patients with ulcerative colitis.

ABSTRACT:

Background

Disturbances in the intestinal microbial community (i.e. dysbiosis) or presence of the microbes with deleterious effects on colonic mucosa has been linked to the pathogenesis of inflammatory bowel diseases. However the role of microbiota in induction and progression of ulcerative colitis (UC) has not yet been fully elucidated.

Methods

Three lines of human microbiota-associated (HMA) mice were established by gavage of colon biopsy from three patients with active UC. The shift in microbial community during its transferring from humans to mice was analyzed by next-generation sequencing using Illumina MiSeq sequencer. Spontaneous or dextran sulfate sodium (DSS)-induced colitis and microbiota composition profiling in germ-free mice and HMA mice over 3-4 generations wer

SUBMITTER: Du Z 

PROVIDER: S-EPMC4687314 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature