The interactive association between heat shock factor 1 and heat shock proteins in primary myocardial cells subjected to heat stress.

ABSTRACT: Heat shock factor 1 (HSF1) is a heat shock transcription factor that rapidly induces heat shock gene transcription following thermal stress. In this study, we subjected primary neonatal rat myocardial cells to heat stress in vitro to create a model system for investigating the trends in expression and association between various heat shock proteins (HSPs) and HSF1 under adverse environmental conditions. After the cells were subjected to heat stress at 42˚C for different periods of time, HSP and HSF1 mRNA and protein levels were detected by qPCR and western blot analysis in the heat-stressed cells. The HSF1 expression levels significantly increased in the cells following 120 min of exposure to heat stess compared to the levels observed at the beginning of heat stress exposure. HSP90 followe

SUBMITTER: Tang S 

PROVIDER: S-EPMC4687434 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature