Epidemiological and virological findings during multiple outbreaks of equine influenza in South America in 2012.

ABSTRACT:

Background

In 2012, equine influenza (EI) virus was confirmed as the cause of outbreaks of respiratory disease in horses throughout South America. In Uruguay and Argentina, hundreds of vaccinated thoroughbred horses in training and racing facilities were clinically affected.

Objective

To characterise the EI viruses detected during the outbreak in Uruguay and Argentina.

Methods

Virus was detected in nasopharyngeal swabs by a pan-reactive influenza type A real-time RT-PCR. The nucleotide sequence of the HA1 gene was determined and analysed phylogenetically using mega 5 software. Amino acid sequences alignments were constructed and virus was antigenically characterised with specific ferret antisera. Paired serum samples were tested by haemagglutination inhibition and sin

SUBMITTER: Perglione CO 

PROVIDER: S-EPMC4687505 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature