Different downstream signalling of CCK1 receptors regulates distinct functions of CCK in pancreatic beta cells.

ABSTRACT:

Background and purpose

Cholecystokinin (CCK) is secreted by intestinal I cells and regulates important metabolic functions. In pancreatic islets, CCK controls beta cell functions primarily through CCK1 receptors, but the signalling pathways downstream of these receptors in pancreatic beta cells are not well defined.

Experimental approach

Apoptosis in pancreatic beta cell apoptosis was evaluated using Hoechst-33342 staining, TUNEL assays and Annexin-V-FITC/PI staining. Insulin secretion and second messenger production were monitored using ELISAs. Protein and phospho-protein levels were determined by Western blotting. A glucose tolerance test was carried out to examine the functions of CCK-8s in streptozotocin-induced diabetic mice.

Key results

The sulfated carboxy-term

SUBMITTER: Ning SL 

PROVIDER: S-EPMC4687794 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature