In Vivo Monosynaptic Excitatory Transmission between Layer 2 Cortical Pyramidal Neurons.

ABSTRACT: Little is known about the properties of monosynaptic connections between identified neurons in vivo. We made multiple (two to four) two-photon targeted whole-cell recordings from neighboring layer 2 mouse somatosensory barrel cortex pyramidal neurons in vivo to investigate excitatory monosynaptic transmission in the hyperpolarized downstate. We report that pyramidal neurons form a sparsely connected (6.7% connectivity) network with an overrepresentation of bidirectional connections. The majority of unitary excitatory postsynaptic potentials were small in amplitude (<0.5 mV), with a small minority >1 mV. The coefficient of variation (CV = 0.74) could largely be explained by the presence of synaptic failures (22%). Both the CV and failure rates were reduced with increasing amplitude. The mea

SUBMITTER: Jouhanneau JS 

PROVIDER: S-EPMC4688033 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature