Th17 cytokines differentiate obesity from obesity-associated type 2 diabetes and promote TNFα production.

ABSTRACT:

Objective

T cell inflammation plays pivotal roles in obesity-associated type 2 diabetes (T2DM). The identification of dominant sources of T cell inflammation in humans remains a significant gap in understanding disease pathogenesis. It was hypothesized that cytokine profiles from circulating T cells identify T cell subsets and T cell cytokines that define T2DM-associated inflammation.

Methods

Multiplex analyses were used to quantify T cell-associated cytokines in αCD3/αCD28-stimulated PBMCs, or B cell-depleted PBMCs, from subjects with T2DM or BMI-matched controls. Cytokine measurements were subjected to multivariate (principal component and partial least squares) analyses. Flow cytometry detected intracellular TNFα in multiple immune cell subsets in the presence/absence of

SUBMITTER: Ip B 

PROVIDER: S-EPMC4688084 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature