MiRNA signature identification of retinoblastoma and the correlations between differentially expressed miRNAs during retinoblastoma progression.

ABSTRACT:

Purpose

Retinoblastoma (RB) is a common pediatric cancer. The study aimed to uncover the mechanisms of RB progression and identify novel therapeutic biomarkers.

Methods

The miRNA expression profile GSE7072, which includes three RB samples and three healthy retina samples, was used. After data normalization using the preprocessCore package, differentially expressed miRNAs (DE-miRs) were selected by the limma package. The targets of the DE-miRs were predicted based on two databases, followed by construction of the miRNA-target network. Pathway enrichment analysis was conducted for the targets of the DE-miRNAs using DAVID. The CTD database was used to predict RB-related genes, followed by clustering analysis using the pvclust package. The correlation network of DE-miRs was esta

SUBMITTER: Yang Y 

PROVIDER: S-EPMC4688417 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature