Breast Cancer Cell Colonization of the Human Bone Marrow Adipose Tissue Niche.

ABSTRACT:

Background/objectives

Bone is a preferred site of breast cancer metastasis, suggesting the presence of tissue-specific features that attract and promote the outgrowth of breast cancer cells. We sought to identify parameters of human bone tissue associated with breast cancer cell osteotropism and colonization in the metastatic niche.

Methods

Migration and colonization patterns of MDA-MB-231-fLuc-EGFP (luciferase-enhanced green fluorescence protein) and MCF-7-fLuc-EGFP breast cancer cells were studied in co-culture with cancellous bone tissue fragments isolated from 14 hip arthroplasties. Breast cancer cell migration into tissues and toward tissue-conditioned medium was measured in Transwell migration chambers using bioluminescence imaging and analyzed as a function of secrete

SUBMITTER: Templeton ZS 

PROVIDER: S-EPMC4688564 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature