Differential expression of a novel colorectal cancer differentiation-related gene in colorectal cancer.

ABSTRACT:

Aim

To investigate SBA2 expression in CRC cell lines and surgical specimens of CRC and autologous healthy mucosa.

Methods

Reverse transcription-polymerase chain reaction (RT-PCR) was used for relative quantification of SBA2 mRNA levels in 4 human CRC cell lines with different grades of differentiation and 30 clinical samples. Normalization of the results was achieved by simultaneous amplification of beta-actin as an internal control.

Results

In the exponential range of amplification, fairly good linearity demonstrated identical amplification efficiency for SBA2 and beta-actin (82%). Markedly lower levels of SBA2 mRNA were detectable in tumors, as compared with the coupled normal counterparts P<0.01). SBA2 expression was significantly (0.01>P < 0.05) correlated with th

SUBMITTER: Li XG 

PROVIDER: S-EPMC4688671 | biostudies-literature | 2001 Aug

REPOSITORIES: biostudies-literature