GPR55 promotes migration and adhesion of colon cancer cells indicating a role in metastasis.

ABSTRACT:

Background and purpose

Tumour cell migration and adhesion constitute essential features of metastasis. G-protein coupled receptor 55 (GPR55), a lysophospholipid receptor, has been shown to play an important role in carcinogenesis. Here, we investigated the involvement of GPR55 in migration and metastasis of colon cancer cells.

Experimental approach

Adhesion and migration assays using the highly metastatic colon cancer cell line HCT116 and an in vivo assay of liver metastasis were performed. The GPR55 antagonist CID16020046, cannabidiol, a putative GPR55 antagonist and GPR55 siRNA were used to block GPR55 activity in HCT116 colon cancer cells.

Key results

HCT116 cells showed a significant decrease in adhesion to endothelial cells and in migration after blockade with CI

SUBMITTER: Kargl J 

PROVIDER: S-EPMC4688947 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature