Origin-Dependent Inverted-Repeat Amplification: Tests of a Model for Inverted DNA Amplification.

ABSTRACT: DNA replication errors are a major driver of evolution--from single nucleotide polymorphisms to large-scale copy number variations (CNVs). Here we test a specific replication-based model to explain the generation of interstitial, inverted triplications. While no genetic information is lost, the novel inversion junctions and increased copy number of the included sequences create the potential for adaptive phenotypes. The model--Origin-Dependent Inverted-Repeat Amplification (ODIRA)-proposes that a replication error at pre-existing short, interrupted, inverted repeats in genomic sequences generates an extrachromosomal, inverted dimeric, autonomously replicating intermediate; subsequent genomic integration of the dimer yields this class of CNV without loss of distal chromosomal sequences. We

SUBMITTER: Brewer BJ 

PROVIDER: S-EPMC4689423 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature