Separation of in-vitro-derived megakaryocytes and platelets using spinning-membrane filtration.

ABSTRACT: In-vitro-derived platelets (PLTs) could potentially overcome problems associated with donated PLTs, including contamination and alloimmunization. Although several groups have produced functional PLTs from stem cells in vitro, the challenge of developing this technology to yield transfusable PLT units has yet to be addressed. The asynchronous nature of in vitro PLT generation makes a single harvest point infeasible for collecting PLTs as soon as they are formed. The current standard of performing manual centrifugations to separate PLTs from nucleated cells at multiple points during culture is labor-intensive, imprecise, and difficult to standardize in accordance with current Good Manufacturing Practices (cGMP). In an effort to develop a more effective method, we adapted a commercially-avail

SUBMITTER: Schlinker AC 

PROVIDER: S-EPMC4689600 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature