Autoantibodies targeting glomerular annexin A2 identify patients with proliferative lupus nephritis.

ABSTRACT:

Purpose

Patients with systemic lupus erythematosus (SLE) frequently develop lupus nephritis (LN), a complication frequently leading to end stage kidney disease. Immune complex deposition in the glomerulus is central to the development of LN. Using a targeted proteomic approach, we tested the hypothesis that autoantibodies targeting glomerular antigens contribute to the development of LN.

Experimental design

Human podocyte and glomerular proteins were separated by SDS-PAGE and immunoblotted with sera from SLE patients with and without LN. The regions of those gels corresponding to reactive bands observed with sera from LN patients were analyzed using LC-MS/MS.

Results

LN reactive bands were seen at approximately 50 kDa in podocyte extracts and between 36 and 50 kDa in

SUBMITTER: Caster DJ 

PROVIDER: S-EPMC4690797 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature