Alteration of the Helicobacter pylori membrane proteome in response to changes in environmental salt concentration.

ABSTRACT:

Purpose

Helicobacter pylori infection and a high dietary salt intake are each risk factors for the development of gastric cancer. We hypothesize that changes in environmental salt concentrations lead to alterations in the H. pylori membrane proteome.

Experimental design

Label-free and iTRAQ methods were used to identify H. pylori proteins that change in abundance in response to alterations in environmental salt concentrations. In addition, we biotinylated intact bacteria that were grown under high- or low-salt conditions, and thereby analyzed salt-induced changes in the abundance of surface-exposed proteins.

Results

Proteins with increased abundance in response to high salt conditions included CagA, the outer membrane protein HopQ, and fibronectin domain-containing pr

SUBMITTER: Voss BJ 

PROVIDER: S-EPMC4690801 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature