Ontology highlight
ABSTRACT:
SUBMITTER: Toledo CM
PROVIDER: S-EPMC4691575 | biostudies-literature | 2015 Dec
REPOSITORIES: biostudies-literature
Cell reports 20151207 11
To identify therapeutic targets for glioblastoma (GBM), we performed genome-wide CRISPR-Cas9 knockout (KO) screens in patient-derived GBM stem-like cells (GSCs) and human neural stem/progenitors (NSCs), non-neoplastic stem cell controls, for genes required for their in vitro growth. Surprisingly, the vast majority GSC-lethal hits were found outside of molecular networks commonly altered in GBM and GSCs (e.g., oncogenic drivers). In vitro and in vivo validation of GSC-specific targets revealed se ...[more]