Subcellular RNA profiling links splicing and nuclear DICER1 to alternative cleavage and polyadenylation.

ABSTRACT: Alternative cleavage and polyadenylation (APA) plays a crucial role in the regulation of gene expression across eukaryotes. Although APA is extensively studied, its regulation within cellular compartments and its physiological impact remains largely enigmatic. Here, we used a rigorous subcellular fractionation approach to compare APA profiles of cytoplasmic and nuclear RNA fractions from human cell lines. This approach allowed us to extract APA isoforms that are subjected to differential regulation and provided us with a platform to interrogate the molecular regulatory pathways that shape APA profiles in different subcellular locations. Here, we show that APA isoforms with shorter 3' UTRs tend to be overrepresented in the cytoplasm and appear to be cell-type-specific events. Nuclear retent

SUBMITTER: Neve J 

PROVIDER: S-EPMC4691748 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature