Cytoplasmic poly(A) binding protein-1 binds to genomically encoded sequences within mammalian mRNAs.

ABSTRACT: The functions of the major mammalian cytoplasmic poly(A) binding protein, PABPC1, have been characterized predominantly in the context of its binding to the 3' poly(A) tails of mRNAs. These interactions play important roles in post-transcriptional gene regulation by enhancing translation and mRNA stability. Here, we performed transcriptome-wide CLIP-seq analysis to identify additional PABPC1 binding sites within genomically encoded mRNA sequences that may impact on gene regulation. From this analysis, we found that PABPC1 binds directly to the canonical polyadenylation signal in thousands of mRNAs in the mouse transcriptome. PABPC1 binding also maps to translation initiation and termination sites bracketing open reading frames, exemplified most dramatically in replication-dependent histone

SUBMITTER: Kini HK 

PROVIDER: S-EPMC4691835 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature