Sequence characterized amplified region marker as a tool for selection of high-artemisinin containing species of Artemisia.

ABSTRACT: Malaria is currently one of the most important causes of mortality in developing countries. High resistance to available antimalarial drugs has been reported frequently, thus it is crucial to focus on the discovery of new antimalarial drugs. Artemisinin, an effective antimalarial medication, is isolated from various Artemisia species. To identify the Artemisia species producing high quantity of artemisinin, eight species of Artemisia were screened with the genetic sequence characterized amplified region (SCAR) marker for higher quantity of artemisinin. The DNA band corresponding to SCAR marker was cloned into pGEM®-T Easy vector and sequenced. The content of artemisinin in tested species was also measured using high-performance liquid chromatography (HPLC) assay. The primers designed for h

SUBMITTER: Asghari M 

PROVIDER: S-EPMC4691966 | biostudies-literature | 2015 Sep-Oct

REPOSITORIES: biostudies-literature