Epigenetic Alterations in Density Selected Human Spermatozoa for Assisted Reproduction.

ABSTRACT: Epidemiological evidence indicates that assisted reproductive technologies (ART) may be associated with several epigenetic diseases such as Beckwith-Wiedemann syndrome (BWS) or Silver-Russell syndrome (SRS). Selection of sperm by density-gradients in ART has improved DNA integrity and sperm quality; however, epigenetic alterations associated with this approach are largely unknown. In the present study, we investigated DNA methylation and histone retention profiles in raw sperm and selected sperm derived from the same individual and separated by using density-gradients. Results from a study group consisting of 93 males demonstrated that both global DNA methylation and histone retention levels decreased in density selected sperm. Compared to unselected raw sperm, histone transition rates dec

SUBMITTER: Yu B 

PROVIDER: S-EPMC4692407 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature