Generation of Prostate Cancer Patient Derived Xenograft Models from Circulating Tumor Cells.

ABSTRACT: Patient derived xenograft (PDX) models are gaining popularity in cancer research and are used for preclinical drug evaluation, biomarker identification, biologic studies, and personalized medicine strategies. Circulating tumor cells (CTC) play a critical role in tumor metastasis and have been isolated from patients with several tumor types. Recently, CTCs have been used to generate PDX experimental models of breast and prostate cancer. This manuscript details the method for the generation of prostate cancer PDX models from CTCs developed by our group. Advantages of this method over conventional PDX models include independence from surgical sample collection and generating experimental models at various disease stages. Density gradient centrifugation followed by red blood cell lysis and flo

SUBMITTER: Williams ES 

PROVIDER: S-EPMC4692658 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature