Identification of Epstein-Barr Virus Replication Proteins in Burkitt's Lymphoma Cells.

ABSTRACT: The working model to describe the mechanisms used to replicate the cancer-associated virus Epstein-Barr virus (EBV) is partly derived from comparisons with other members of the Herpes virus family. Many genes within the EBV genome are homologous across the herpes virus family. Published transcriptome data for the EBV genome during its lytic replication cycle show extensive transcription, but the identification of the proteins is limited. We have taken a global proteomics approach to identify viral proteins that are expressed during the EBV lytic replication cycle. We combined an enrichment method to isolate cells undergoing EBV lytic replication with SILAC-labeling coupled to mass-spectrometry and identified viral and host proteins expressed during the OPEN ACCESS Pathogens 2015, 4 740 EBV

SUBMITTER: Traylen C 

PROVIDER: S-EPMC4693162 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature