Thrombospondin-1, -2 and -5 have differential effects on vascular smooth muscle cell physiology.

ABSTRACT:

Introduction

The thrombospondins (TSPs) are matricellular proteins that exert multifunctional effects by binding cytokines, cell-surface receptors and other proteins. TSPs play important roles in vascular pathobiology and are all expressed in arterial lesions. The differential effects of TSP-1, -2, and -5 represent a gap in knowledge in vascular smooth muscle cell (VSMC) physiology. Our objective is to determine if structural differences of the TSPs imparted different effects on VSMC functions critical to the formation of neointimal hyperplasia. We hypothesize that TSP-1 and -2 induce similar patterns of migration, proliferation and gene expression, while the effects of TSP-5 are different.

Methods

Human aortic VSMC chemotaxis was tested for TSP-2 and TSP-5 (1-40 μg/mL), and

SUBMITTER: Helkin A 

PROVIDER: S-EPMC4694046 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature