MiR-1 induces growth arrest and apoptosis in malignant mesothelioma.

ABSTRACT:

Background

We investigated microRNA expression profiles of malignant pleural mesothelioma (MPM) specimens to identify novel microRNA that are potentially involved in the oncogenic transformation of human pleural cells.

Methods

microRNA microarray transcriptional profiling studies of 25 MPM primary tumors were performed. We used normal pleural tissue from an unmatched patient cohort as normal comparators. To confirm microarray data, we used real-time quantitative polymerase chain reaction. Representative cell lines H513 and H2052 were used in functional analyses of miR-1.

Results

In addition to several novel MPM-associated microRNAs, we observed that the expression level of miR-1 was significantly lower in tumors as compared with normal pleural specimens. Subsequently,

SUBMITTER: Xu Y 

PROVIDER: S-EPMC4694093 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature