Effect of Smac in combination with cisplatin on esophageal cancer cell line ECA109.

ABSTRACT:

Objective

This study was to investigate inhibiting effect of structurally unique Second mitochondria-derived activator of caspase (Smac) in combination with cisplatin on esophageal cancer cell line ECA109.

Methods

PcDNA3.1-Smac (ECA109/Smac group), pcDNA3.1 (ECA109/neo group) and PBS (ECA109 or control group) were transfected into ECA109 cells respectively, and transfected cells which expressed Smac stably were got. Smac protein expression was analyzed by Western blot. The invasive ability of cells was examined. Flow cytometry was used to analyze apoptosis induced by cisplatin with Annexin V/PI double staining technique.

Results

Smac gene was successfully transfected into ECA109 cell, over-expression of Smac could decrease cell invasive ability obviously compared to c

SUBMITTER: Hou L 

PROVIDER: S-EPMC4694360 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature