In vivo imaging and tracking of host-microbiota interactions via metabolic labeling of gut anaerobic bacteria.

ABSTRACT: The intestine is densely populated by anaerobic commensal bacteria. These microorganisms shape immune system development, but understanding of host-commensal interactions is hampered by a lack of tools for studying the anaerobic intestinal environment. We applied metabolic oligosaccharide engineering and bioorthogonal click chemistry to label various commensal anaerobes, including Bacteroides fragilis, a common and immunologically important commensal. We studied the dissemination of B. fragilis after acute peritonitis and characterized the interactions of the intact microbe and its polysaccharide components in myeloid and B cell lineages. We were able to assess the distribution and colonization of labeled B. fragilis along the intestine, as well as niche competition after coadministration

SUBMITTER: Geva-Zatorsky N 

PROVIDER: S-EPMC4694768 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature