Patient-derived cell models as preclinical tools for genome-directed targeted therapy.

ABSTRACT:

Background

In this study, we established patient-derived tumor cell (PDC) models using tissues collected from patients with metastatic cancer and assessed whether these models could be used as a tool for genome-based cancer treatment.

Methods

PDCs were isolated and cultured from malignant effusions including ascites and pleural fluid. Pathological examination, immunohistochemical analysis, and genomic profiling were performed to compare the histological and genomic features of primary tumors, PDCs. An exploratory gene expression profiling assay was performed to further characterize PDCs.

Results

From January 2012 to May 2013, 176 samples from patients with metastatic cancer were collected. PDC models were successfully established in 130 (73.6%) samples. The median tim

SUBMITTER: Lee JY 

PROVIDER: S-EPMC4694854 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature