Genome-wide analysis of DNA methylation and gene expression patterns in purified, uncultured human liver cells and activated hepatic stellate cells.

ABSTRACT:

Background & aims

Liver fibrogenesis - scarring of the liver that can lead to cirrhosis and liver cancer - is characterized by hepatocyte impairment, capillarization of liver sinusoidal endothelial cells (LSECs) and hepatic stellate cell (HSC) activation. To date, the molecular determinants of a healthy human liver cell phenotype remain largely uncharacterized. Here, we assess the transcriptome and the genome-wide promoter methylome specific for purified, non-cultured human hepatocytes, LSECs and HSCs, and investigate the nature of epigenetic changes accompanying transcriptional changes associated with activation of HSCs.

Material and methods

Gene expression profile and promoter methylome of purified, uncultured human liver cells and culture-activated HSCs were respectively

SUBMITTER: El Taghdouini A 

PROVIDER: S-EPMC4694948 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature