Tumour exosomes display differential mechanical and complement activation properties dependent on malignant state: implications in endothelial leakiness.

ABSTRACT:

Background

Exosomes have been implicated in tumour progression and metastatic spread. Little is known of the effect of mechanical and innate immune interactions of malignant cell-derived exosomes on endothelial integrity, which may relate to increased extravasation of circulating tumour cells and, therefore, increased metastatic spread.

Methods

Exosomes isolated from non-malignant immortalized HCV-29 and isogenic malignant non-metastatic T24 and malignant metastatic FL3 bladder cells were characterized by nanoparticle tracking analysis and quantitative nanomechanical mapping atomic force microscopy (QNM AFM) to determine size and nanomechanical properties. Effect of HCV-29, T24 and FL3 exosomes on human umbilical vein endothelial cell (HUVEC) monolayer integrity was determin

SUBMITTER: Whitehead B 

PROVIDER: S-EPMC4695623 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature