Next-Generation Sequencing in Clinical Molecular Diagnostics of Cancer: Advantages and Challenges.

ABSTRACT: The application of next-generation sequencing (NGS) to characterize cancer genomes has resulted in the discovery of numerous genetic markers. Consequently, the number of markers that warrant routine screening in molecular diagnostic laboratories, often from limited tumor material, has increased. This increased demand has been difficult to manage by traditional low- and/or medium-throughput sequencing platforms. Massively parallel sequencing capabilities of NGS provide a much-needed alternative for mutation screening in multiple genes with a single low investment of DNA. However, implementation of NGS technologies, most of which are for research use only (RUO), in a diagnostic laboratory, needs extensive validation in order to establish Clinical Laboratory Improvement Amendments (CLIA) and

SUBMITTER: Luthra R 

PROVIDER: S-EPMC4695874 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature