Analysis of the mitochondrial maxicircle of Trypanosoma lewisi, a neglected human pathogen.

ABSTRACT:

Background

The haemoflagellate Trypanosoma lewisi is a kinetoplastid parasite which, as it has been recently reported to cause human disease, deserves increased attention. Characteristic features of all kinetoplastid flagellates are a uniquely structured mitochondrial DNA or kinetoplast, comprised of a network of catenated DNA circles, and RNA editing of mitochondrial transcripts. The aim of this study was to describe the kinetoplast DNA of T. lewisi.

Methods/results

In this study, purified kinetoplast DNA from T. lewisi was sequenced using high-throughput sequencing in combination with sequencing of PCR amplicons. This allowed the assembly of the T. lewisi kinetoplast maxicircle DNA, which is a homologue of the mitochondrial genome in other eukaryotes. The assembly of 23,74

SUBMITTER: Lin RH 

PROVIDER: S-EPMC4696184 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature