Transfection of Sertoli cells with androgen receptor alters gene expression without androgen stimulation.

ABSTRACT:

Background

Androgens play an important role for the development of male fertility and gained interest as growth and survival factors for certain types of cancer. Androgens act via the androgen receptor (AR/Ar), which is involved in various cell biological processes such as sex differentiation. To study the functional mechanisms of androgen action, cell culture systems and AR-transfected cell lines are needed. Transfection of AR into cell lines and subsequent gene expression analysis after androgen treatment is well established to investigate the molecular biology of target cells. However, it remains unclear how the transfection with AR itself can modulate the gene expression even without androgen stimulation. Therefore, we transfected Ar-deficient rat Sertoli cells 93RS2 by electro

SUBMITTER: Fietz D 

PROVIDER: S-EPMC4696255 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature