Differential Ly6C Expression after Renal Ischemia-Reperfusion Identifies Unique Macrophage Populations.

ABSTRACT: Macrophages are a heterogeneous cell type implicated in injury, repair, and fibrosis after AKI, but the macrophage population associated with each phase is unclear. In this study, we used a renal bilateral ischemia-reperfusion injury mouse model to identify unique monocyte/macrophage populations by differential expression of Ly6C in CD11b(+) cells and to define the function of these cells in the pathophysiology of disease on the basis of microarray gene signatures and reduction strategies. Macrophage populations were isolated from kidney homogenates by fluorescence-activated cell sorting for whole genome microarray analysis. The CD11b(+)/Ly6C(high) population associated with the onset of renal injury and increase in proinflammatory cytokines, whereas the CD11b(+)/Ly6C(intermediate) populat

SUBMITTER: Clements M 

PROVIDER: S-EPMC4696575 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature