MALDI-TOF Mass Spectrometry: A Powerful Tool for Clinical Microbiology at Hopital Principal de Dakar, Senegal (West Africa).

ABSTRACT: Our team in Europe has developed the routine clinical laboratory identification of microorganisms by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). To evaluate the utility of MALDI-TOF MS in tropical Africa in collaboration with local teams, we installed an apparatus in the Hôpital Principal de Dakar (Senegal), performed routine identification of isolates, and confirmed or completed their identification in France. In the case of discordance or a lack of identification, molecular biology was performed. Overall, 153/191 (80.1%) and 174/191 (91.1%) isolates yielded an accurate and concordant identification for the species and genus, respectively, with the 2 different MALDI-TOF MSs in Dakar and Marseille. The 10 most common bacteria, representing

SUBMITTER: Lo CI 

PROVIDER: S-EPMC4696746 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature