Metformin Improves Diabetic Bone Health by Re-Balancing Catabolism and Nitrogen Disposal.

ABSTRACT:

Objective

Metformin, a leading drug used to treat diabetic patients, is reported to benefit bone homeostasis under hyperglycemia in animal models. However, both the molecular targets and the biological pathways affected by metformin in bone are not well identified or characterized. The objective of this study is to investigate the bioengergeric pathways affected by metformin in bone marrow cells of mice.

Materials and methods

Metabolite levels were examined in bone marrow samples extracted from metformin or PBS -treated healthy (Wild type) and hyperglycemic (diabetic) mice using liquid chromatography-mass spectrometry (LC-MS)-based metabolomics. We applied an untargeted high performance LC-MS approach which combined multimode chromatography (ion exchange, reversed phase and

SUBMITTER: Li X 

PROVIDER: S-EPMC4696809 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature