Dynamics of protein kinases: insights from nuclear magnetic resonance.

ABSTRACT: Protein kinases are ubiquitous enzymes with critical roles in cellular processes and pathology. As a result, researchers have studied their activity and regulatory mechanisms extensively. Thousands of X-ray structures give snapshots of the architectures of protein kinases in various states of activation and ligand binding. However, the extent of and manner by which protein motions and conformational dynamics underlie the function and regulation of these important enzymes is not well understood. Nuclear magnetic resonance (NMR) methods provide complementary information about protein conformation and dynamics in solution. However, until recently, the large size of these enzymes prevented researchers from using these methods with kinases. Developments in transverse relaxation-optimized spectr

SUBMITTER: Xiao Y 

PROVIDER: S-EPMC4697736 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature