Gender-specific differences in diabetic neuropathy in BTBR ob/ob mice.

ABSTRACT:

Aims

To identify a female mouse model of diabetic peripheral neuropathy (DPN), we characterized DPN in female BTBR ob/ob mice and compared their phenotype to non-diabetic and gender-matched controls. We also identified dysregulated genes and pathways in sciatic nerve (SCN) and dorsal root ganglia (DRG) of female BTBR ob/ob mice to determine potential DPN mechanisms.

Methods

Terminal neuropathy phenotyping consisted of examining latency to heat stimuli, sciatic motor and sural sensory nerve conduction velocities (NCV), and intraepidermal nerve fiber (IENF) density. For gene expression profiling, DRG and SCN were dissected, RNA was isolated and processed using microarray technology and differentially expressed genes were identified.

Results

Similar motor and sensory NCV

SUBMITTER: O'Brien PD 

PROVIDER: S-EPMC4698064 | biostudies-literature | 2016 Jan-Feb

REPOSITORIES: biostudies-literature