The Notch ligand E3 ligase, Mind Bomb1, regulates glutamate receptor localization in Drosophila.

ABSTRACT: The postsynaptic density (PSD) is a protein-rich network important for the localization of postsynaptic glutamate receptors (GluRs) and for signaling downstream of these receptors. Although hundreds of PSD proteins have been identified, many are functionally uncharacterized. We conducted a reverse genetic screen for mutations that affected GluR localization using Drosophila genes that encode homologs of mammalian PSD proteins. 42.8% of the mutants analyzed exhibited a significant change in GluR localization at the third instar larval neuromuscular junction (NMJ), a model synapse that expresses homologs of AMPA receptors. We identified the E3 ubiquitin ligase, Mib1, which promotes Notch signaling, as a regulator of synaptic GluR localization. Mib1 positively regulates the localization of th

SUBMITTER: Sturgeon M 

PROVIDER: S-EPMC4698086 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature