Rapid, comprehensive, and affordable mycobacterial diagnosis with whole-genome sequencing: a prospective study.

ABSTRACT:

Background

Slow and cumbersome laboratory diagnostics for Mycobacterium tuberculosis complex (MTBC) risk delayed treatment and poor patient outcomes. Whole-genome sequencing (WGS) could potentially provide a rapid and comprehensive diagnostic solution. In this prospective study, we compare real-time WGS with routine MTBC diagnostic workflows.

Methods

We compared sequencing mycobacteria from all newly positive liquid cultures with routine laboratory diagnostic workflows across eight laboratories in Europe and North America for diagnostic accuracy, processing times, and cost between Sept 6, 2013, and April 14, 2014. We sequenced specimens once using local Illumina MiSeq platforms and processed data centrally using a semi-automated bioinformatics pipeline. We identified species

SUBMITTER: Pankhurst LJ 

PROVIDER: S-EPMC4698465 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature