Amy63, a novel type of marine bacterial multifunctional enzyme possessing amylase, agarase and carrageenase activities.

ABSTRACT: A multifunctional enzyme is one that performs multiple physiological functions, thus benefiting the organism. Characterization of multifunctional enzymes is important for researchers to understand how organisms adapt to different environmental challenges. In the present study, we report the discovery of a novel multifunctional enzyme Amy63 produced by marine bacterium Vibrio alginolyticus 63. Remarkably, Amy63 possesses amylase, agarase and carrageenase activities. Amy63 is a substrate promiscuous α-amylase, with the substrate priority order of starch, carrageenan and agar. Amy63 maintains considerable amylase, carrageenase and agarase activities and stabilities at wide temperature and pH ranges, and optimum activities are detected at temperature of 60 °C and pH of 6.0, respectively. Moreo

SUBMITTER: Liu G 

PROVIDER: S-EPMC4698717 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature