Epigenetic modification with trichostatin A does not correct specific errors of somatic cell nuclear transfer at the transcriptomic level; highlighting the non-random nature of oocyte-mediated reprogramming errors.

ABSTRACT:

Background

The limited duration and compromised efficiency of oocyte-mediated reprogramming, which occurs during the early hours following somatic cell nuclear transfer (SCNT), may significantly interfere with epigenetic reprogramming, contributing to the high incidence of ill/fatal transcriptional phenotypes and physiological anomalies occurring later during pre- and post-implantation events. A potent histone deacetylase inhibitor, trichostatin A (TSA), was used to understand the effects of assisted epigenetic modifications on transcriptional profiles of SCNT blastocysts and to identify specific or categories of genes affected.

Results

TSA improved the yield and quality of in vitro embryo development compared to control (CTR-NT). Significance analysis of microarray results

SUBMITTER: Hosseini SM 

PROVIDER: S-EPMC4698792 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature