Effect of Stratification on Surface Properties of Corneal Epithelial Cells.

ABSTRACT:

Purpose

The purpose of this study was to determine the influence of mucin expression in an immortalized human corneal epithelial cell line (hTCEpi) on the surface properties of cells, such as wettability, contact angle, and surface heterogeneity.

Methods

hTCEpi cells were cultured to confluence in serum-free medium. The medium was then replaced by stratification medium to induce mucin biosynthesis. The mucin expression profile was analyzed using quantitative PCR and Western blotting. Contact angles were measured using a two-immiscible liquid method, and contact angle hysteresis was evaluated by tilting the apparatus and recording advancing and receding contact angles. The spatial distribution of mucins was evaluated with fluorescently labeled lectin.

Results

hTCEpi ce

SUBMITTER: Yanez-Soto B 

PROVIDER: S-EPMC4699408 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature