Physical Factors Affecting Outflow Facility Measurements in Mice.

ABSTRACT:

Purpose

Mice are commonly used to study conventional outflow physiology. This study examined how physical factors (hydration, temperature, and anterior chamber [AC] deepening) influence ocular perfusion measurements in mice.

Methods

Outflow facility (C) and pressure-independent outflow (Fu) were assessed by multilevel constant pressure perfusion of enucleated eyes from C57BL/6 mice. To examine the effect of hydration, seven eyes were perfused at room temperature, either immersed to the limbus in saline and covered with wet tissue paper or exposed to room air. Temperature effects were examined in 12 eyes immersed in saline at 20 °C or 35 °C. Anterior chamber deepening was examined in 10 eyes with the cannula tip placed in the anterior versus posterior chamber (PC). Posterior

SUBMITTER: Boussommier-Calleja A 

PROVIDER: S-EPMC4699419 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature