Characterization and Quantification of Innate Lymphoid Cell Subsets in Human Lung.

ABSTRACT:

Background

Innate lymphoid cells (ILC) are a new family of innate immune cells that have emerged as important regulators of tissue homeostasis and inflammation. However, limited data are available concerning the relative abundance and characteristics of ILC in the human lung.

Methods

The aim of this study was to characterize and enumerate the different ILC subsets in human lung by multi-color flow cytometry.

Results

Within the CD45+ Lin- CD127+ pulmonary ILC population, we identified group 1 (ILC1), group 2 (ILC2) and group 3 (ILC3) innate lymphoid cells using specific surface markers (i.e. IL12Rβ2, CRTH2 and CD117 respectively) and key transcription factors (i.e. T-bet, GATA-3 and RORγT respectively). Based on the presence of NKp44, ILC3 were further subdivided in na

SUBMITTER: De Grove KC 

PROVIDER: S-EPMC4699688 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature