The Effect of In Vitro Cultivation on the Transcriptome of Adult Brugia malayi.

ABSTRACT:

Background

Filarial nematodes cause serious and debilitating infections in human populations of tropical countries, contributing to an entrenched cycle of poverty. Only one human filarial parasite, Brugia malayi, can be maintained in rodents in the laboratory setting. It has been a widely used model organism in experiments that employ culture systems, the impact of which on the worms is unknown.

Methodology/principal findings

Using Illumina RNA sequencing, we characterized changes in gene expression upon in vitro maintenance of adult B. malayi female worms at four time points: immediately upon removal from the host, immediately after receipt following shipment, and after 48 h and 5 days in liquid culture media. The dramatic environmental change and the 24 h time lapse betwee

SUBMITTER: Ballesteros C 

PROVIDER: S-EPMC4699822 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature