Identification of early transcriptome signatures in placenta exposed to insulin and obesity.

ABSTRACT:

Objective

The purpose of this study was to investigate the effects of insulin on human placental transcriptome and biological processes in first-trimester pregnancy.

Study design

Maternal plasma and placenta villous tissue were obtained at the time of voluntary termination of pregnancy (7-12 weeks) from 17 lean (body mass index, 20.9±1.5 kg/m2) and 18 obese (body mass index, 33.5±2.6 kg/m2) women. Trophoblast cells were immediately isolated for in vitro treatment with insulin or vehicle. Patterns of global gene expression were analyzed using genome microarray profiling after hybridization to Human Gene 1.1 ST and real time reverse transcription-polymerase chain reaction.

Results

The global trophoblast transcriptome was qualitatively separated in insulin-treated vs unt

SUBMITTER: Lassance L 

PROVIDER: S-EPMC4700505 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature