Concurrent isolation of lymphocytes and granulocytes using prefocused free flow acoustophoresis.

ABSTRACT: Microchip-based free flow acoustophoresis (FFA) in combination with two-dimensional cell prefocusing enables concurrent multiple target outlet fractionation of leukocytes into subpopulations (lymphocytes, monocytes, and granulocytes); we report on this method here. We also observed significantly increased accuracy in size-based fractionation of microbeads as compared to previously presented FFA multiple outlet systems. Fluorescence microscopy illustrates the importance of two-dimensional prefocusing where a sample mixture of 3, 7, and 10 μm beads are separated into well-confined particle streams and collected in their respective target outlets. Flow cytometry data for lymphocytes and granulocytes, respectively, in their corresponding outlets verify concurrent isolation of leukocyte subpopu

SUBMITTER: Grenvall C 

PROVIDER: S-EPMC4700836 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature