Predicting RAD-seq Marker Numbers across the Eukaryotic Tree of Life.

ABSTRACT: High-throughput sequencing of reduced representation libraries obtained through digestion with restriction enzymes--generically known as restriction site associated DNA sequencing (RAD-seq)--is a common strategy to generate genome-wide genotypic and sequence data from eukaryotes. A critical design element of any RAD-seq study is knowledge of the approximate number of genetic markers that can be obtained for a taxon using different restriction enzymes, as this number determines the scope of a project, and ultimately defines its success. This number can only be directly determined if a reference genome sequence is available, or it can be estimated if the genome size and restriction recognition sequence probabilities are known. However, both scenarios are uncommon for nonmodel species. Here,

SUBMITTER: Herrera S 

PROVIDER: S-EPMC4700943 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature