Determining the Position of Storks on the Phylogenetic Tree of Waterbirds by Retroposon Insertion Analysis.

ABSTRACT: Despite many studies on avian phylogenetics in recent decades that used morphology, mitochondrial genomes, and/or nuclear genes, the phylogenetic positions of several birds (e.g., storks) remain unsettled. In addition to the aforementioned approaches, analysis of retroposon insertions, which are nearly homoplasy-free phylogenetic markers, has also been used in avian phylogenetics. However, the first step in the analysis of retroposon insertions, that is, isolation of retroposons from genomic libraries, is a costly and time-consuming procedure. Therefore, we developed a high-throughput and cost-effective protocol to collect retroposon insertion information based on next-generation sequencing technology, which we call here the STRONG (Screening of Transposons Obtained by Next Generation Sequ

SUBMITTER: Kuramoto T 

PROVIDER: S-EPMC4700946 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature