A Graphene-Based Biosensing Platform Based on Regulated Release of an Aptameric DNA Biosensor.

ABSTRACT: A novel biosensing platform was developed by integrating an aptamer-based DNA biosensor with graphene oxide (GO) for rapid and facile detection of adenosine triphosphate (ATP, as a model target). The DNA biosensor, which is locked by GO, is designed to contain two sensing modules that include recognition site for ATP and self-replication track that yields the nicking domain for Nt.BbvCI. By taking advantage of the different binding affinity of single-stranded DNA, double-stranded DNA and aptamer-target complex toward GO, the DNA biosensor could be efficiently released from GO in the presence of target with the help of a complementary DNA strand (CPDNA) that partially hybridizes to the DNA biosensor. Then, the polymerization/nicking enzyme synergetic isothermal amplification could be trigge

SUBMITTER: Mao Y 

PROVIDER: S-EPMC4701278 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature