In vivo visualization of embryonic stem cell survival, proliferation, and migration after cardiac delivery.

ABSTRACT:

Background

Recent studies have shown that stem cell therapy can promote tissue regeneration; however, monitoring stem cells in vivo remains problematic owing to limitations of conventional histological assays and imaging modalities.

Methods and results

Murine embryonic stem (ES) cells were stably transduced with a lentiviral vector carrying a novel triple-fusion (TF) reporter gene that consists of firefly luciferase, monomeric red fluorescence protein, and truncated thymidine kinase (fluc-mrfp-ttk). ES cell viability, proliferation, and differentiation ability were not adversely affected by either reporter genes or reporter probes compared with nontransduced control cells (P=NS). Afterward, 1x10(7) of ES cells carrying the TF reporter gene (ES-TF) were injected into the myoc

SUBMITTER: Cao F 

PROVIDER: S-EPMC4701384 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature